pcr for pyrosequencing analyses Search Results


pcr product  (Thermo Fisher)
99
Thermo Fisher pcr product
Pcr Product, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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5.16s rrna amplification by pcr for  (Pyrosequencing Inc)
90
Pyrosequencing Inc 5.16s rrna amplification by pcr for
5.16s Rrna Amplification By Pcr For, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pcr amplification  (EpigenDx)
93
EpigenDx pcr amplification
Pcr Amplification, supplied by EpigenDx, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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emulsion pcr  (Pyrosequencing Inc)
90
Pyrosequencing Inc emulsion pcr
Emulsion Pcr, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rt-pcr-pyrosequencing assay  (Pyrosequencing Inc)
90
Pyrosequencing Inc rt-pcr-pyrosequencing assay
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Rt Pcr Pyrosequencing Assay, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rt-pcr-pyrosequencing assay/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
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single-stranded biotinylated pcr products  (BIOTAGE)
90
BIOTAGE single-stranded biotinylated pcr products
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Single Stranded Biotinylated Pcr Products, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
single-stranded biotinylated pcr products - by Bioz Stars, 2026-04
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454-pyrosequencing  (Pyrosequencing Inc)
90
Pyrosequencing Inc 454-pyrosequencing
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
454 Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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raindance pcr products  (RainDance Technologies)
90
RainDance Technologies raindance pcr products
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Raindance Pcr Products, supplied by RainDance Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
raindance pcr products - by Bioz Stars, 2026-04
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dpcr pyrosequencing  (Pyrosequencing Inc)
90
Pyrosequencing Inc dpcr pyrosequencing
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Dpcr Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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bisulfite-pcr pyrosequencing  (Pyrosequencing Inc)
90
Pyrosequencing Inc bisulfite-pcr pyrosequencing
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Bisulfite Pcr Pyrosequencing, supplied by Pyrosequencing Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/bisulfite-pcr pyrosequencing/product/Pyrosequencing Inc
Average 90 stars, based on 1 article reviews
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cfx-96 sequence detector  (Bio-Rad)
90
Bio-Rad cfx-96 sequence detector
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Cfx 96 Sequence Detector, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/cfx-96 sequence detector/product/Bio-Rad
Average 90 stars, based on 1 article reviews
cfx-96 sequence detector - by Bioz Stars, 2026-04
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qpcr primers  (BIOTAGE)
90
BIOTAGE qpcr primers
Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the <t>S</t> <t>protein</t> and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.
Qpcr Primers, supplied by BIOTAGE, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the S protein and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.

Journal: Journal of Medical Virology

Article Title: A pyrosequencing protocol for rapid identification of SARS‐CoV‐2 variants

doi: 10.1002/jmv.27770

Figure Lengend Snippet: Schematic representation of the location of forward and reverse PCR primers (arrows) used to amplify four regions of the S gene of SARS‐CoV‐2. PCR 1 and PCR 2 target sequences corresponding to the N‐terminal domain (NTD) of the S protein and overlap by 11 bases. PCR 2 resides within the receptor‐binding domain (RBD), and PCR 4 overlies the junction between the S1 and S2 subunits (S1/S2). Common S gene mutations reported in SARS‐CoV‐2 variants, detectable by pyrosequencing the PCR products, using sequencing primers or forward PCR primers as listed in Table , are indicated in the respective boxes. Forty‐two mutations detected in specimens analyzed in the current study appear in bold type. Other rare, novel mutations may be detected within targeted sequences but are not listed.

Article Snippet: The current study describes the development of a reverse transcription (RT)‐PCR‐pyrosequencing assay targeting >65 spike protein gene ( S ) mutations of SARS‐CoV‐2, which permits differentiation of commonly reported variants currently circulating in the United States with a high degree of confidence.

Techniques: Binding Assay, Sequencing